The test has been shown to be non-reactive with the following bacteria (at 1×108 organisms per sample):
Acinetobacter calcoaceticus, Aeromonashydrophila subsp. hydrophila, Bacillus subtilis, Burkholderia cepacia, Citrobacterfreudii, Citrobacterkoseri, Escherichia coli, Enterobacter cloacae, Klebsiellaoxytoca, Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas putida, Pseudomonas stutzeri, Ralstoniapickettii, Raoultellaterrigena, Streptococcus pyrogenes, Yersinia ruckeri..
Staphylococcus aureus and Legionella pneumophila serogroups 4 and 7 in samples at concentrations higher than 1×108 organisms per sample may produce weak positive results. These concentrations are higher than would be expected to be present in normal water samples.
Legionella pneumophila serogroups 4 & 7 cross react with the test at concentrations >109 CFU/sample. The test does not react with any other L. pneumophila serogroups or L. species tested.